![]() crassa are produced by four different mechanisms that include a distinct combination of factors, including Dicers, Argonaute protein QDE-2, the exonuclease QIP, and the RNAse III domain-containing MRP元 protein. However, the biosynthesis mechanism of fungal miRNAs are different from the animal and plant miRNAs. For instance, the miRNA precursors of plants and animals have a typical hairpin structure that is similar to those of fungi. The characteristics of fungal milRNAs are similar to those of plant and animal miRNAs. The first miRNA-like fungal RNAs (milRNAs) were discovered in Neurospora crassa ( Lee et al., 2010) and the milRNAs have been subsequently identified in other filamentous fungi and basidiomycetes ( Kang et al., 2013 Wang et al., 2021). Numerous miRNAs of plants and animals have been identified to date. MicroRNAs (miRNAs) are small non-coding RNA molecules that are 18–24 nt long, play important regulatory roles in gene regulation, and influence various biological processes in plants and animals. This study provides novel insights into the molecular mechanism underlying the development of fruit bodies in P. These findings indicated that milR20 plays a negative role in the development of P. The results demonstrated that the overexpression of milR20 reduced the growth rate of mycelia and prolonged the development of the fruit bodies, while milR20 silencing had an opposite effect. The function of milR20, which targeted pheromone A receptor g8971 and was involved in the MAPK signaling pathway, was further verified by overexpression and silencing in P. Comparison of the DEGs and DEMs across the different development stages revealed that DEMs and its target DEGs involved in the mitogen-activated protein kinase (MAPK) signaling pathway, protein processing in endoplasmic reticulum, endocytosis, aminoacyl-tRNA biosynthesis, RNA transport, and other metabolism pathways, which may play important roles in the development of the fruit bodies of P. The total number of differentially expressed genes (DEGs) and differentially expressed milRNAs (DEMs) at different stages of development was determined to be 7,934 and 20, respectively. The genes that play a crucial role in the expression and function of milRNAs were identified and subsequently expressed and silenced at different stages of development. In this study, the role of milRNAs in the development of fruit bodies of Pleurotus cornucopiae was studied by comparative analyses of the mRNAs and milRNAs at different stages of development. ![]() The mechanism underlying the development of fruit bodies in edible mushroom is a widely studied topic. 4College of Life Sciences, Shanxi Normal University, Taiyuan, China.3State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Beijing, China.2Key Laboratory of Microbial Resources, Ministry of Agriculture and Rural Affairs, Beijing, China.1Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China.Yuhui Qi 1,2,3, Chenyang Huang 1,2,3, Mengran Zhao 1,2,3, Xiangli Wu 1,2,3, Guangyu Li 1,2,3, Yingjie Zhang 1,2,3,4 and Lijiao Zhang 1,2,3 *
0 Comments
Leave a Reply. |